<code lang="Wfg5t"></code> Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

Comparative Study
. 2009:4:127-35.
doi: 10.2147/copd.s4639. Epub 2009 Apr 15.

Reticular basement membrane in asthma and COPD: similar thickness, yet different composition

Jeroen J W Liesker  1 , Nick H Ten Hacken, Mieke Zeinstra-Smith, Steven R Rutgers, Dirkje S Postma, Wim Timens
Affiliations
Comparative Study

Reticular basement membrane in asthma and COPD: similar thickness, yet different composition

Jeroen J W Liesker (V体育平台登录) et al. Int J Chron Obstruct Pulmon Dis. 2009.

Abstract

Background: Reticular basement membrane (RBM) thickening has been variably associated with asthma and chronic obstructive pulmonary disease (COPD). Even if RBM thickness is similar in both diseases, its composition might still differ. VSports手机版.

Objective: To assess whether RBM thickness and composition differ between asthma and COPD. V体育安卓版.

Methods: We investigated 24 allergic asthmatics (forced expiratory volume in one second [FEV(1)] 92% predicted), and 17 nonallergic COPD patients (FEV(1) 60% predicted), and for each group a control group of similar age and smoking habits (12 and 10 persons, respectively). Snap-frozen sections of bronchial biopsies were stained with hematoxylin/eosin and for collagen I, III, IV, V, laminin and tenascin. RBM thickening was assessed by digital image analysis. Relative staining intensity of each matrix component was determined V体育ios版. .

Results: Mean (SD) RBM thickness was not significantly different between asthma and COPD 5. 5 (1 VSports最新版本. 3) vs 6. 0 (1. 8) microm, but significantly larger than in their healthy counterparts, ie, 4. 7 (0. 9) and 4. 8 (1. 2) microm, respectively. Collagen I and laminin stained significantly stronger in asthma than in COPD. Tenascin stained stronger in asthma than in healthy controls of similar age, and stronger in COPD controls than in asthma controls (p < 0. 05). .

Conclusion: RBM thickening occurs both in asthma and COPD. We provide supportive evidence that its composition differs in asthma and COPD V体育平台登录. .

PubMed Disclaimer

Figures

Figure 1
Figure 1
Mean basement membrane thickness for asthma, COPD, and healthy controls of similar age. Reticular basement membrane thickness in central airway wall biopsies from 24 patients with asthma, 12 age-matched healthy controls of asthma, 17 patients with COPD and 10 age- and pack year-matched healthy controls of COPD. Notes: Solid lines are means; *p < 0.05. Abbreviation: COPD, chronic obstructive pulmonary disease.
Figure 2
Figure 2
Reticular basement membrane composition. Relative staining density of collagen I, laminin, collagen IV, and tenascin in the reticular basement membrane of airway wall biopsies in asthma and COPD patients, and their healthy matched control subject. Results of collagen III and V are not shown as there were no significant differences between any of the groups. Notes: *p < 0.05, #p = 0.08. Abbreviation: COPD, chronic obstructive pulmonary disease.
Figure 3
Figure 3
Collagen I staining. Immunohistochemical staining of bronchial biopsies for collagen I (immunoperoxidase, original magnification × 200) showed, a uniform red-brown-stained band beneath the epithelial layer in asthma (A left panel). In contrast to asthma, this is significantly less intense in COPD (p < 0.05, B right panel). Abbreviation: COPD, chronic obstructive pulmonary disease.
See this image and copyright information in PMC

References

    1. Dunnill MS, Massarella GR, Anderson JA. A comparison of the quantitative anatomy of the bronchi in normal subjects, in status asthmaticus, in chronic bronchitis, and in emphysema. Thorax. 1969;24(2):176–179. - "VSports app下载" PMC - PubMed
    1. Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma. From bronchoconstriction to airways inflammation and remodeling. Am J Respir Crit Care Med. 2000;161(5):1720–1745. - PubMed
    1. Kasahara K, Shiba K, Ozawa T, Okuda K, Adachi M. Correlation between the bronchial subepithelial layer and whole airway wall thickness in patients with asthma. Thorax. 2002;57(3):242–246. - "V体育官网" PMC - PubMed
    1. James AL, Maxwell PS, Pearce-Pinto G, Elliot JG, Carroll NG. The relationship of reticular basement membrane thickness to airway wall remodeling in asthma. Am J Respir Crit Care Med. 2002;166(12 Pt 1):1590–1595. - PubMed
    1. Jeffery PK, Wardlaw AJ, Nelson FC, Collins JV, Kay AB. Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity. Am Rev Respir Dis. 1989;140(6):1745–1753. - PubMed

Publication types

V体育ios版 - MeSH terms