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. 2008 Jul 4;26(29-30):3608-16.
doi: 10.1016/j.vaccine.2008.04.074. Epub 2008 May 19.

Evaluation of systemic and mucosal anti-HPV16 and anti-HPV18 antibody responses from vaccinated women

Troy J Kemp  1 , Alfonso García-Piñeres, Roni T Falk, Sylviane Poncelet, Francis Dessy, Sandra L Giannini, Ana Cecilia Rodriguez, Carolina Porras, Rolando Herrero, Allan Hildesheim, Ligia A Pinto; Costa Rica Vaccine Trial (CVT) Group
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"V体育平台登录" Evaluation of systemic and mucosal anti-HPV16 and anti-HPV18 antibody responses from vaccinated women

"VSports在线直播" Troy J Kemp et al. Vaccine. .

VSports app下载 - Abstract

Ideal methods to monitor HPV neutralizing antibodies induced by vaccination have not been established yet. Here, we evaluated systemic and cervical antibody levels induced by HPV16/18 AS04-adjuvanted vaccine (GlaxoSmithKline Biologicals) using a secreted alkaline phosphatase neutralization assay (SEAP-NA) and enzyme-linked immunosorbent assay (ELISA). Serum and cervical secretions from 50 vaccinated women were used to assess (1) overall assay reproducibility; (2) inter-assay and inter-specimen correlation; (3) correlations between month 1 and month 12 titers. Strong correlations between SEAP-NA and ELISA were observed (serum anti-HPV16/18, rho=0. 91/0. 85; cervix anti-HPV16/18, rho=0. 84/0. 89) VSports手机版. Systemic and cervical antibody measures also correlated well (rho range: 0. 64-0. 75); except at mid-cycle (rho range: 0. 28-0. 65). Correlations between antibody levels at 1 and 12 months following the start of vaccination were poor (rho range: 0. 16-0. 38). In conclusion, HPV16/18 VLP-based ELISA is a reliable and valid method to monitor anti-HPV16/18 neutralizing potential for the first year following vaccination; however, additional studies will be required to better define the effects of the time on cycle and patterns of antibody response over time following vaccination. .

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Figure 1
Figure 1
SEAP-NA and ELISA anti-HPV16 and 18 titers were correlated with each other among serum and cervical secretions. (A) HPV16 SEAP-NA correlated with HPV16 ELISA using serum samples. (B) HPV18 SEAP-NA correlated with HPV18 ELISA using serum samples. (C) HPV16 SEAP-NA correlated with HPV16 ELISA using cervical secretions. (D) HPV18 SEAP-NA correlated with HPV18 ELISA using cervical secretions. Within each graph the total number of samples used in the analysis is represented by ‘N’, and the Spearman rank correlation coefficient and p-value is also indicated on each graph.
Figure 2
Figure 2
Correlations calculated between serum anti-HPV16 and 18 antibody responses and cervical secretion anti-HPV16 and 18 antibody responses. (A) Serum anti-HPV16 antibody titers correlated with cervical secretion anti-HPV16 antibody titers using the HPV16 L1 VLP-based ELISA. (B) Serum anti-HPV18 antibody titers correlated with cervical secretion anti-HPV18 antibody titers using the HPV18 L1 VLP-based ELISA. (C) Serum anti-HPV16 antibody titers correlated with cervical secretion anti-HPV16 antibody titers using the SEAP-NA. (D) Serum anti-HPV18 antibody titers correlated with cervical secretion anti-HPV18 antibody titers using the SEAP-NA. Within each graph the total number of samples used in the analysis is represented by ‘N’, and the Spearman rank correlation coefficient and p-value is also indicated on each graph.
Figure 3
Figure 3
Anti-HPV16 and 18 antibody responses were correlated with each other within the same assay and specimen type. (A) Serum anti-HPV16 antibody titers were correlated with serum anti-HPV18 antibody titers using the titers calculated from the ELISA. (B) Serum anti-HPV16 antibody titers were correlated with serum anti-HPV18 antibody titers using the titers calculated from the SEAP-NA. (C) Cervical secretion anti-HPV16 antibody titers were correlated with cervical secretion anti-HPV18 antibody titers calculated from the ELISA results. (D) Cervical secretion anti-HPV16 antibody titers were correlated with cervical secretion anti-HPV18 antibody titers calculated from the SEAP-NA results. Within each graph the total number of samples used in the analysis is represented by ‘N’, and the Spearman rank correlation coefficient and p-value is also indicated on each graph.
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"VSports最新版本" References

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