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. 1991 May 1;173(5):1213-25.
doi: 10.1084/jem.173.5.1213.

"V体育平台登录" Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow

R R Hardy  1 , C E Carmack, S A Shinton, J D Kemp, K Hayakawa
Affiliations

Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow (VSports手机版)

VSports - R R Hardy et al. J Exp Med. .

Abstract

We have resolved B220+ IgM- B-lineage cells in mouse bone marrow into four fractions based on differential cell surface expression of determinants recognized by S7 (leukosialin, CD43), BP-1, and 30F1 (heat stable antigen). Functional differences among these fractions can be correlated with Ig gene rearrangement status. The largest fraction, lacking S7, consists of pre-B cells whereas the others, expressing S7, include B lineage cells before pre-B. These S7+ fractions, provisionally termed Fr. A, Fr. B, and Fr. C, can differentiate in a stromal layer culture system. Phenotypic alteration during such culture suggests an ordering of these stages from Fr. A to Fr. B to Fr. C and thence to S7- pre-B cells. Using polymerase chain reaction amplification with pairs of oligonucleotide primers for regions 5' of JH1, DFL16 VSports手机版. 1, and Jk1, we find that the Ig genes of Fr. A are in germline configuration, whereas Fr. B and C are pro-B cell stages with increasing D-J rearrangement, but no V-D-J. Finally, functional analysis demonstrates that the proliferative response to IL-7, an early B lineage growth factor, is restricted to S7+ stages and, furthermore, that an additional, cell contact-mediated signal is essential for survival of Fr. A. .

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References (VSports注册入口)

    1. J Immunol. 1987 Sep 1;139(5):1718-26 - VSports最新版本 - PubMed
    1. Science. 1980 Sep 5;209(4461):1128-32 - PubMed (VSports手机版)
    1. J Immunol. 1988 Nov 1;141(9):2912-7 - PubMed
    1. Blood. 1988 May;71(5):1226-33 - PubMed
    1. J Immunol. 1987 Apr 1;138(7):2013-8 - PubMed

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