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Review
. 2008 Jan;21(1):232-52.
doi: 10.1021/tx700292a. Epub 2007 Dec 12.

V体育安卓版 - Biological properties of single chemical-DNA adducts: a twenty year perspective

James C Delaney  1 , John M Essigmann
Affiliations
Review

Biological properties of single chemical-DNA adducts: a twenty year perspective (VSports注册入口)

James C Delaney et al. Chem Res Toxicol. 2008 Jan.

Abstract

The genome and its nucleotide precursor pool are under sustained attack by radiation, reactive oxygen and nitrogen species, chemical carcinogens, hydrolytic reactions, and certain drugs. As a result, a large and heterogeneous population of damaged nucleotides forms in all cells. Some of the lesions are repaired, but for those that remain, there can be serious biological consequences. For example, lesions that form in DNA can lead to altered gene expression, mutation, and death VSports手机版. This perspective examines systems developed over the past 20 years to study the biological properties of single DNA lesions. .

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VSports注册入口 - Figures

Figure 1
Figure 1
Evolution for understanding polymerase and repair contributions to lesion mutagenesis and bypass.
Figure 2
Figure 2
REAP assay for determining the mutation frequency and specificity of lesions (right) and CRAB assay for determining the replication blocking power of lesions (left).
Figure 3
Figure 3
Lesions analyzed by the REAP and CRAB assays.
See this image and copyright information in PMC

V体育ios版 - References

    1. Basu AK, Essigmann JM. Site-specifically modified oligodeoxynucleotides as probes for the structural and biological effects of DNA-damaging agents. Chem Res Toxicol. 1988;1:1–18. - PubMed
    1. Neeley WL, Essigmann JM. Mechanisms of formation, genotoxicity, and mutation of guanine oxidation products. Chem Res Toxicol. 2006;19:491–505. - "VSports注册入口" PubMed
    1. Smith HO, Hutchison CA, III, Pfannkoch C, Venter JC. Generating a synthetic genome by whole genome assembly: φX174 Bacteriophage from synthetic oligonucleotides. Proc Natl Acad Sci USA. 2003;100:15440–15445. - PMC - PubMed
    1. Kodumal SJ, Patel KG, Reid R, Menzella HG, Welch M, Santi DV. Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster. Proc Natl Acad Sci USA. 2004;101:15573–15578. - PMC - PubMed
    1. Fowler KW, Büchi G, Essigmann JM. Synthesis and characterization of an oligonucleotide containing a carcinogen-modified base: O6-methylguanine. J Am Chem Soc. 1982;104:1050–1054.

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