V体育安卓版 - Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil VSports app下载. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2007 Mar;73(6):2009-12.
doi: 10.1128/AEM.02561-06. Epub 2007 Jan 26.

Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples

Petra Louis  1 , Harry J Flint
Affiliations

"VSports手机版" Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples

Petra Louis et al. Appl Environ Microbiol. 2007 Mar.

Abstract

We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota VSports手机版. .

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Nucleic acid and amino acid sequence alignments of primer binding sites of degenerate primers BCoATscrF and BCoATscrR. Butyryl-CoA CoA transferases: R. hom., R. hominis A2-183; E. hal., E. hallii L2-7; A. cac. I, A. caccae L1-92; F. prau., F. prausnitzii A2-165. Putative acetyl-CoA hydrolase: D. haf., D. hafniense. 4-Hydroxybutyrate CoA transferases: C. klu., C. kluyveri; C. tet., C. tetani; A. cac. II, A. caccae L1-92; C. am., C. aminobutyricum (for accession numbers, see the text). Primer binding sites correspond to nucleotide positions 217 to 249 for primer BCoATscrF and 748 to 773 for primer BCoATscrR of the butyryl-CoA CoA transferase gene from R. hominis A2-183. (*) The sequence for primer BCoATscrR is given in reverse to enable comparison to the alignment. Amino acids encoded by the primer sequence are indicated. Arrows indicate primer direction. Black shading indicates positions identical to the butyryl-CoA CoA transferase consensus, and gray shading indicates matches due to degeneracy of the primers. I, inosine; N, A, C, G or T; S, C or G; W, A or T; Y, T or C.
FIG. 2.
FIG. 2.
Quantification of butyryl-CoA CoA transferase genes within samples from fermentors inoculated with human fecal slurries (start) from two donors grown at two pH values and in the presence of 0.6% peptide as described by Walker et al. (16). Data for quantification of bacterial groups based on FISH are taken from reference . Gray bars, F. prausnitzii group; black bars, Roseburia spp. and E. rectale group; hatched bars, other clostridial cluster XIVa bacteria; stippled bars, results of quantification by real-time PCR, expressed as the number of CoA transferase genes detected per 16S rRNA gene.
See this image and copyright information in PMC

References

    1. Acinas, S. G., L. A. Marcelino, V. Klepac-Ceraj, and M. F. Polz. 2004. Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons. J. Bacteriol. 186:2629-2635. - PMC - PubMed
    1. Aminov, R. I., A. W. Walker, S. H. Duncan, H. J. Harmsen, G. W. Welling, and H. J. Flint. 2006. Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale. Appl. Environ. Microbiol. 72:6371-6376. - PMC - PubMed
    1. Barcenilla, A. 1999. Ph.D. thesis. Robert Gordon University, Aberdeen, United Kingdom.
    1. Barcenilla, A., S. E. Pryde, J. C. Martin, S. H. Duncan, C. S. Stewart, C. Henderson, and H. J. Flint. 2000. Phylogenetic relationships of butyrate-producing bacteria from the human gut. Appl. Environ. Microbiol. 66:1654-1661. - PMC (V体育安卓版) - PubMed
    1. Belenguer, A., S. H. Duncan, A. G. Calder, G. Holtrop, P. Louis, G. E. Lobley, and H. J. Flint. 2006. Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut. Appl. Environ. Microbiol. 72:3593-3599. - PMC - PubMed

VSports在线直播 - Publication types

MeSH terms (V体育2025版)

LinkOut - more resources