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Comparative Study
. 2006 Jul;74(7):4014-20.
doi: 10.1128/IAI.01237-05.

"V体育平台登录" Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis

Sonia Manco  1 , Fidelma Hernon, Hasan Yesilkaya, James C Paton, Peter W Andrew, Aras Kadioglu
Affiliations
Comparative Study

Pneumococcal neuraminidases A and B both have essential roles during infection of the respiratory tract and sepsis

"V体育官网" Sonia Manco et al. Infect Immun. 2006 Jul.

Abstract (VSports注册入口)

We examined the role of the neuraminidases NanA and NanB in colonization and infection in the upper and lower respiratory tract by Streptococcus pneumoniae, as well as the role of these neuraminidases in the onset and development of septicemia following both intranasal and intravenous infection. We demonstrated for the first time using outbred MF1 mouse models of infection that both NanA and NanB were essential for the successful colonization and infection of the upper and lower respiratory tract, respectively, as well as pneumococcal survival in nonmucosal sites, such as the blood. Our studies have shown that in vivo a neuraminidase A mutant is cleared from the nasopharynx, trachea, and lungs within 12 h postinfection, while a neuraminidase B mutant persists but does not increase in either the nasopharynx, trachea, or lungs. We also demonstrated both neuraminidase mutants were unable to cause sepsis following intranasal infections. When administered intravenously, however, both mutants survived initially but were unable to persist in the blood beyond 48 h postinfection and were progressively cleared. The work presented here demonstrates the importance of pneumococcal neuraminidase A and for the first time neuraminidase B in the development of upper and lower respiratory tract infection and sepsis VSports手机版. .

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Figures

FIG. 1.
FIG. 1.
Time course of change in numbers of S. pneumoniae in nasopharynx (A), trachea (B), and lungs (C) after intranasal infection of mice with 106 CFU of the wild type (squares), NanA− (solid triangles), or NanB− (open triangles). n = 5 mice per each time point per each strain used; error bars indicate SEM.
FIG. 2.
FIG. 2.
Time course of change in numbers of S. pneumoniae in blood infected by the intravenous route. Mice were infected with 105 CFU of the wild type (squares), NanA− (solid triangles), or NanB− (open triangles). Tail bleeds were taken from 10 mice for each strain at 3, 6, 24, and 48 h post-intravenous infection. Error bars indicate SEM.
FIG. 3.
FIG. 3.
Light microscopy of lung tissue from mice infected intranasally with 106 CFU S. pneumoniae NanB− at 24 h postinfection (A) or the wild type at 24 h postinfection (B). Magnification, ×250. In panel A, the long arrow indicates a mild peribronchial cellular infiltrate, the double arrow indicates mild levels of bronchial wall hypertrophy, and the short arrow shows alveolar spaces. In panel B, the double arrow indicates hypertrophy of inflamed bronchiole walls, and the long arrow indicates severe multifocal peribronchial infiltration of inflammatory cells and extensive infiltration of lung parenchyma. Exudate is represented by a short arrow.
See this image and copyright information in PMC

References

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