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. 2006 Jan 22;273(1583):251-6.
doi: 10.1098/rspb.2005.3301.

Experimental evolution of resistance to an antimicrobial peptide (VSports在线直播)

Gabriel G Perron  1 , Michael Zasloff, Graham Bell
Affiliations

Experimental evolution of resistance to an antimicrobial peptide

Gabriel G Perron et al. Proc Biol Sci. .

V体育ios版 - Abstract

A novel class of antibiotics based on the antimicrobial properties of immune peptides of multicellular organisms is attracting increasing interest as a major weapon against resistant microbes. It has been claimed that cationic antimicrobial peptides exploit fundamental features of the bacterial cell so that resistance is much less likely to evolve than in the case of conventional antibiotics. Population models of the evolutionary genetics of resistance have cast doubt on this claim VSports手机版. We document the experimental evolution of resistance to a cationic antimicrobial peptide through continued selection in the laboratory. In this selection experiment, 22/24 lineages of Escherichia coli and Pseudomonas fluorescens independently evolved heritable mechanisms of resistance to pexiganan, an analogue of magainin, when propagated in medium supplemented with this antimicrobial peptide for 600-700 generations. .

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Figures

Figure 1
Figure 1
Growth of Pseudomonas fluorescens in relation to concentration of pexiganan. Mean (±s.e.) growth is measured as (a) absorbance before selection and (b) after selection and presents data from positive selection lines and control selection lines for wild-type (SBW25) and mutator (SBW25 Δmuts) genotypes. Note that lineages were not assayed at concentrations above 512 μg ml−1 before selection, since growth was totally inhibited.
Figure 2
Figure 2
Growth of Escherichia coli in relation to concentration of pexiganan. Mean (±s.e.) growth is measured as absorbance (a) before selection and (b) after selection and presents data from positive selection lines and control selection lines for wild-type (PS8) and mutator (PS8 mutL) genotypes. Note that lineages were not assayed at concentrations above 512 μg ml−1 before selection, since growth was totally inhibited.
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