Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil VSports app下载. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

Comparative Study
. 2006 Feb;97(2):106-12.
doi: 10.1111/j.1349-7006.2006.00147.x.

Phenotypic characterization of endometrial stromal sarcoma of the uterus

Kazumi Yamada  1 , Junko Kano, Hajime Tsunoda, Hiroyuki Yoshikawa, Chigusa Okubo, Tadashi Ishiyama, Masayuki Noguchi
Affiliations
Comparative Study

Phenotypic characterization of endometrial stromal sarcoma of the uterus

Kazumi Yamada et al. Cancer Sci. 2006 Feb.

Abstract

Endometrial stromal sarcoma (ESS) of the uterus is a rare uterine malignancy that has not been characterized in detail. To characterize the phenotype of ESS of the uterus, we extracted RNA from ESS and the stroma of normal endometrium using a tissue microdissection system and compared the expression profiles in the two tissues. After suppression subtractive hybridization and differential screening, we detected the metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) gene as one of the major genes upregulated in ESS, and a full-length placental cDNA clone (CS0DI066YJ10) as one of the major genes downregulated. The results were confirmed by in situ hybridization in four resected specimens of ESS and 36 biopsy specimens of normal endometrial tissue. All ESS (4/4) and all cases of endometrial stromal cells in the proliferative phase (13/13) were positive for MALAT-1, but samples of normal stroma in the secretory phase and menopausal state included some that were negative or weakly positive for MALAT-1 (5/13 and 3/10, respectively). In contrast, all ESS and 12 of 13 cases of stromal cells in the proliferative phase were negative for the full-length placental cDNA clone but 10 of 13 cases of endometrial stromal cells in the secretory phase were positive for transcripts of the gene (P < 0. 05) VSports手机版. These results indicated that endometrial stromal cells have different phenotypic characteristics between proliferative and secretory phases and the tumor cells of ESS have the phenotypic character of endometrial stromal cells in the proliferative phase. .

PubMed Disclaimer

Figures (VSports在线直播)

Figure 1
Figure 1
Histology of (a) endometrial stromal sarcoma and (b) normal endometrium in secretory phase (HE, ×200). Frozen specimens of two cases were examined by suppression subtractive hybridization.
Figure 2
Figure 2
Histology of normal endometrium. (a) Frozen section after laser capture microdissection, and (b) captured cells on a capsure (×100).
Figure 3
Figure 3
In situ hybridization of metastasis associated lung adenocarcinoma transcript 1 (MALAT‐1) mRNA. (a) The nuclei of the endometrial sarcoma, (b) both epithelial and stromal cells in proliferative phase and (c) epithelial cells in secretory phase were positive for MALAT‐1. However, stromal cells in secretory phase were negative for the gene (×200).
Figure 4
Figure 4
Immunohistochemistry of granulysin protein. Infiltrating lymphocytes (arrows) were positive for the antigranulysin antibody, but stromal cells were negative (×400).
Figure 5
Figure 5
In situ hybridization of CS0DI066YJ10 mRNA. (a) Endometrial stromal sarcoma, (b) endometrial tissue in proliferative phase and (c) epithelial cells in secretory phase were negative for CS0DI066YJ10. The stromal cells in secretory phase (c) and decidual cells in the placenta (d) were selectively positive for the gene (a–c, ×200; d, ×100).
See this image and copyright information in PMC

References

    1. De Fusco PA, Gaffy TA, Cha SS et al. Endometrial stromal sarcoma: review of Mayo Clinic experience, 1945–1980. Gynecol Oncol 1989; 35: 8–14. - PubMed
    1. Layfield LJ, Liu K, Dodge R, Barsky SH. Uterine smooth muscle tumors: utility of classification by proliferation, ploidy, and prognostic markers versus traditional histopathology. Arch Pathol Lab Med 2000; 124: 221–7. - PubMed
    1. Chang KL, Crabtree GS, Hendrickson MR et al. Primary uterine endometrial stromal neoplasms: a clinicopathologic study of 117 cases. Am J Surg Pathol 1990; 14: 415–38. - PubMed
    1. Bodner K, Bodner‐Adler B, Obermair A et al. Prognostic parameters in endometrial stromal sarcoma: a clinicopathological study in 31 patients. Gynecol Oncol 2001; 81: 160–5. - PubMed
    1. Chu MC, Mor G, Shwartz PE et al. Low‐grade endometrial stromal sarcoma: hormonal aspects. Gynecol Oncol 2003; 90: 170–6. - PubMed

Publication types

"V体育平台登录" MeSH terms