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Comparative Study
. 2005 Jun 14;11(22):3485-9.
doi: 10.3748/wjg.v11.i22.3485.

"V体育官网" Comparative proteome analysis of untreated and Helicobacter pylori-treated HepG2

Yan Zhang  1 , Xue-Gong Fan, Ren Chen, Zhi-Qiang Xiao, Xue-Ping Feng, Xue-Fei Tian, Zhao-Hui Chen
Affiliations
Comparative Study

Comparative proteome analysis of untreated and Helicobacter pylori-treated HepG2

Yan Zhang et al. World J Gastroenterol. .

Abstract

Aim: To investigate the pathological effect of Helicobacter pylori (H pylori) on human hepatic cells, proteomic methods were used to find and to identify proteins that were overexpressed in HepG2 cells treated by H pylori VSports手机版. .

Methods: H pylori was co-cultured with HepG2 for 6 h. Two-dimensional gel electrophoresis was used to gain the protein expression pattern of untreated and H pylori-treated HepG2. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry V体育安卓版. .

Results: Seven proteins, which were up-regulated in H pylori-treated HepG2 cells, were identified. These proteins included integrin beta-1, protein kinase C alpha, LIM/homeobox protein Lhx1, eIF-2-beta, MAP kinase kinase 3, PINCH protein and Ras-related protein Rab-37, which involved in transcription regulation, signal transduction, metabolism and so on. V体育ios版.

Conclusion: H pylori may exert the pathological effect on HepG2 cells by up-regulating the expression of some proteins. VSports最新版本.

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Figures

Figure 1
Figure 1
2-DE maps of untreated (A) and H pylori-treated HepG2 (B) cell proteins. The proteins from cells were extracted and separated on a pH 3-10 IPG strip, followed by a 12.5% SDS-polyacrylamide gel and silver staining. Spots that were found to be overexpressed have been annotated.
Figure 2
Figure 2
Peptide mass fingerprinting of the spot 10. The spot was in-gel digested with trypsin. After desalting, the peptide mixture was analyzed by MALDI-TOF-MS.
See this image and copyright information in PMC

References

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