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Comparative Study
. 1992 May 15;256(5059):1014-8.
doi: 10.1126/science.1589769.

"V体育官网入口" Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos

M A Blanar  1 , W J Rutter
Affiliations
Comparative Study

Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos

M A Blanar et al. Science. .

Abstract

A facile method for isolating genes that encode interacting proteins has been developed with a polypeptide probe that contains an amino-terminal extension with recognition sites for a monoclonal antibody, a specific endopeptidase, and a site-specific protein kinase. This probe, containing the basic region-leucine zipper dimerization motif of c-Fos, was used to screen a complementary DNA library. A complementary DNA that encoded a member of the basic-helix-loop-helix-zipper (bHLH-Zip) family of proteins was isolated VSports手机版. The complementary DNA-encoded polypeptide FIP (Fos interacting protein) bound to oligonucleotide probes that contained DNA binding motifs for other HLH proteins. When cotransfected with c-Fos, FIP stimulated transcription of an AP-1-responsive promoter. .

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