Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or VSports app下载. mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2005 Apr 22;308(5721):523-9.
doi: 10.1126/science.1105809.

Causal protein-signaling networks derived from multiparameter single-cell data

Karen Sachs  1 , Omar Perez, Dana Pe'er, Douglas A Lauffenburger, Garry P Nolan
Affiliations

Causal protein-signaling networks derived from multiparameter single-cell data

Karen Sachs et al. Science. .

Erratum in

  • Science. 2005 Aug 19;309(5738):1187

Abstract

Machine learning was applied for the automated derivation of causal influences in cellular signaling networks. This derivation relied on the simultaneous measurement of multiple phosphorylated protein and phospholipid components in thousands of individual primary human immune system cells. Perturbing these cells with molecular interventions drove the ordering of connections between pathway components, wherein Bayesian network computational methods automatically elucidated most of the traditionally reported signaling relationships and predicted novel interpathway network causalities, which we verified experimentally. Reconstruction of network models from physiologically relevant primary single cells might be applied to understanding native-state tissue signaling biology, complex drug actions, and dysfunctional signaling in diseased cells VSports手机版. .

PubMed Disclaimer

Comment in

Publication types

MeSH terms

"VSports手机版" Substances