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. 2005 Mar;71(3):1276-82.
doi: 10.1128/AEM.71.3.1276-1282.2005.

"VSports在线直播" Influence of starvation on potential ammonia-oxidizing activity and amoA mRNA levels of Nitrosospira briensis

Annette Bollmann  1 , Ingo Schmidt, Aaron M Saunders, Mette H Nicolaisen
Affiliations

"V体育ios版" Influence of starvation on potential ammonia-oxidizing activity and amoA mRNA levels of Nitrosospira briensis

Annette Bollmann et al. Appl Environ Microbiol. 2005 Mar.

Abstract

The effect of short-term ammonia starvation on Nitrosospira briensis was investigated. The ammonia-oxidizing activity was determined in a concentrated cell suspension with a NOx biosensor. The apparent half-saturation constant [Km(app)] value of the NH3 oxidation of N. briensis was 3 microM NH3 for cultures grown both in continuous and batch cultures as determined by a NOx biosensor. Cells grown on the wall of the vessel had a lower Km(app) value of 1. 8 microM NH3. Nonstarving cultures of N. briensis showed potential ammonia-oxidizing activities of between 200 to 250 microM N h(-1), and this activity decreased only slowly during starvation up to 10 days. Within 10 min after the addition of fresh NH4+, 100% activity was regained. Parallel with activity measurements, amoA mRNA and 16S rRNA were investigated. No changes were observed in the 16S rRNA, but a relative decrease of amoA mRNA was observed during the starvation period. During resuscitation, an increase in amoA mRNA expression was detected simultaneously. The patterns of the soluble protein fraction of a 2-week-starved culture of N. briensis showed only small differences in comparison to a nonstarved control. From these results we conclude that N. briensis cells remain in a state allowing fast recovery of ammonia-oxidizing activity after addition of NH4+ to a starved culture. Maintaining cells in this kind of active state could be the survival strategy of ammonia-oxidizing bacteria in nature under fluctuating NH4+ availability VSports手机版. .

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Figures

FIG. 1.
FIG. 1.
NH4+ dynamics (•) and potential ammonia-oxidizing activity (○) of a coculture of N. briensis and N. winogradskyi during growth and starvation (reactor 1).
FIG. 2.
FIG. 2.
amoA mRNA and 16S rRNA RT-PCR products of N. briensis from the reactor 1 during starvation for NH4+.
FIG. 3.
FIG. 3.
NH4+ dynamics (•) and potential ammonia-oxidizing activity (○) of a coculture of N. briensis and N. winogradskyi during growth, starvation, and resuscitation (reactor 2). A, overall picture; B, detailed graph for the first hours of resuscitation.
FIG. 4.
FIG. 4.
Ammonia-oxidizing activity over time in the concentrated samples used to measure the potential ammonia-oxidizing activity at the different time points during growth, starvation, and resuscitation (reactor 2). The ammonia-oxidizing activity was calculated for every minute as the slope of the NO2−/NO3− production within the 2 min before and after each time point. ▪, during growth; •, 3 days starved; ○, 7 days starved; ▴, after 10 min fresh NH4+; ▵, after 4 h fresh NH4+.
FIG. 5.
FIG. 5.
amoA mRNA and 16S rRNA RT-PCR products of N. briensis from the reactor 2 during starvation and resuscitation.
FIG. 6.
FIG. 6.
Comparison of the protein pattern of the soluble protein fraction of a growing and a 2-week-starved culture of N. briensis. Circles with solid lines indicate protein spots disappearing and circles with dashed lines indicate spots where protein spots are appearing during starvation. MW, molecular weight.
FIG. 7.
FIG. 7.
NH4+ dynamics and potential ammonia-oxidizing activity of a coculture of N. briensis and N. winogradskyi during resuscitation after starvation treated with and without acetylene before addition of fresh NH4+ (reactor 3). □, NH4+ (without acetylene treatment); ▪, NH4+ (after acetylene treatment); ▵, potential ammonia-oxidizing activity (without acetylene treatment); ▴, potential ammonia-oxidizing activity (after acetylene treatment).
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References

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