Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official VSports app下载. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely V体育官网. .

. 2003 Nov;41(11):5245-9.
doi: 10.1128/JCM.41.11.5245-5249.2003.

"V体育平台登录" Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR

Robert M Wadowsky  1 , Stella Laus, Michael Green, Steven A Webber, David Rowe
Affiliations

Measurement of Epstein-Barr virus DNA loads in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR

Robert M Wadowsky et al. J Clin Microbiol. 2003 Nov.

Abstract

Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r(2) > 0 VSports手机版. 900), whereas the plasma and PBL loads correlated poorly (r(2) = 0. 512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load. .

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Correlation of EBV DNA load in plasma and whole-blood samples (n = 59 pairs) measured by TaqMan PCR. Values less than 50 copies/ml were coded as 50 copies/ml. The diagonal line represents theoretical, perfect agreement between viral loads in plasma and whole blood.
See this image and copyright information in PMC

V体育ios版 - References

    1. Fan, H., S. A. Schichman, L. J. Swinnen, J. M. Nicholls, P. A. Eagan, M. Luther, and M. L. Gulley. 2001. Analytic validation of a competitive polymerase chain reaction assay for measuring Epstein-Barr viral load. Diagn. Mol. Pathol. 10:255-264. - PubMed
    1. Green, M., J. Bueno, D. Rowe, G. Mazariegos, L. Qu, K. Abu-Almagd, and J. Reyes. 2000. Predictive negative value of persistent low Epstein-Barr virus viral load after intestinal transplantation in children. Transplantation 70:593-596. - PubMed
    1. Green, M., T. V. Cacciarelli, G. B. Mazariegos, L. Sigurdsson, L. Qu, D. T. Rowe, and J. Reyes. 1998. Serial measurement of Epstein-Barr viral load in peripheral blood in pediatric liver transplant recipients during treatment for posttransplant lymphoproliferative disease. Transplantation 66:1641-1644. - PubMed
    1. Kimura, H., M. Morita, Y. Yabuta, K. Kuzushima, K. Kato, S. Kojima, T. Matsuyama, and T. Morishima. 1999. Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J. Clin. Microbiol. 37:132-136. - PMC - PubMed
    1. Leung, E., B. K. Shenton, G. Jackson, F. K. Gould, C. Yap, and D. Talbot. 2002. Use of real-time PCR to measure Epstein-Barr virus genomes in whole blood. J. Immunol. Methods 270:259-267. - PubMed

"VSports app下载" MeSH terms

LinkOut - more resources