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. 2003 Oct;110(2):242-9.
doi: 10.1046/j.1365-2567.2003.01732.x.

Induction of a protective capsular polysaccharide antibody response to a multiepitope DNA vaccine encoding a peptide mimic of meningococcal serogroup C capsular polysaccharide

Deborah M Prinz  1 , S Louise Smithson, Thomas Kieber-Emmons, M A Julie Westerink
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Induction of a protective capsular polysaccharide antibody response to a multiepitope DNA vaccine encoding a peptide mimic of meningococcal serogroup C capsular polysaccharide (VSports最新版本)

Deborah M Prinz et al. Immunology. 2003 Oct.

"VSports" Abstract

Systemic infection by encapsulated organisms, such as Neisseria meningitidis, is a major cause of morbidity and mortality worldwide, especially in individuals less than 2 years of age. Antibodies directed at the capsular polysaccharide are shown to be protective against disease by inducing complement-dependent bactericidal activity. The current polysaccharide vaccine has been shown to be poorly immunogenic in high-risk groups and this is probably related to its T-independent properties. An alternative approach to eliciting a T-dependent serum immunoglobulin G (IgG) antibody response to encapsulated pathogens is DNA vaccination. We assessed the immunogenicity of a multiepitope DNA vaccine encoding a T-cell helper epitope and a peptide mimic of N. meningitidis serogroup C. The DNA construct induced a significant anti-polysaccharide antibody response that was bactericidal VSports手机版. Mice immunized with the DNA construct were subsequently protected against challenge with a lethal dose of N. meningitidis serogroup C. .

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Figures

Figure 1
Figure 1
Mice (n = 6) were immunized with 100 µg of DNA and 40 µg of aluminum phosphate gel adjuvant on days 0, 21, and 63. Positive controls were immunized with 5 µg of meningococcal serogroup C capsular polysaccharide (MCPS) on day 0. The anti-MCPS immunoglobulin response was determined by enzyme-linked immunosorbent assay (ELISA). The geometric mean immunoglobulin M (IgM) antibody titre of each group is represented by a column. *Statistical significance of groups compared with the negative control (P ≤ 0·05).
Figure 2
Figure 2
Functional activity in postimmune sera was determined by serum bactericidal assays on day 56. Geometric mean bactericidal titres for each group are indicated by columns; individual mouse titres are denoted by circles. Some circles may be representative of more than one mouse in a group. *Statistical significance of groups compared with the negative control (P ≤ 0·05).
Figure 3
Figure 3
Mice immunized with DNA and meningococcal serogroup C capsular polysaccharide (MCPS) were challenged with a lethal dose of Neisseria meningitidis serogroup C strain 35E. Ninety-six hours postchallenge, sera were collected from survivors and anti-MCPS IgG1, IgG2a, IgG2b, and IgG3 were determined by enzyme-linked immunosorbent assay (ELISA). Geometric mean pre- and postchallenge antibody titres for each isotype are represented by columns; individual mouse titres are denoted by circles. Some circles may be representative of more than one mouse. *Statistical significance of the test group compared with controls (P ≤ 0·05).
Figure 3
Figure 3
Mice immunized with DNA and meningococcal serogroup C capsular polysaccharide (MCPS) were challenged with a lethal dose of Neisseria meningitidis serogroup C strain 35E. Ninety-six hours postchallenge, sera were collected from survivors and anti-MCPS IgG1, IgG2a, IgG2b, and IgG3 were determined by enzyme-linked immunosorbent assay (ELISA). Geometric mean pre- and postchallenge antibody titres for each isotype are represented by columns; individual mouse titres are denoted by circles. Some circles may be representative of more than one mouse. *Statistical significance of the test group compared with controls (P ≤ 0·05).
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References (V体育安卓版)

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