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. 1992 Oct 1;89(19):9171-5.
doi: 10.1073/pnas.89.19.9171.

In vitro differentiation of embryonic stem cells into lymphocyte precursors able to generate T and B lymphocytes in vivo (V体育官网)

J C Gutierrez-Ramos  1 , R Palacios
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In vitro differentiation of embryonic stem cells into lymphocyte precursors able to generate T and B lymphocytes in vivo

"V体育平台登录" J C Gutierrez-Ramos et al. Proc Natl Acad Sci U S A. .

Abstract

Embryonic stem cells can be induced in vitro, by coculture with the stromal line RP. 0. 10 and a mixture of interleukins 3, 6, and 7, to differentiate into T (Joro75+) and B (B-220+) lymphocyte progenitors and other (Thy-1+, PgP-1+, c-kit+, Joro75-, B-220-, F4/80-, Mac-1-) hemopoietic precursors. The progeny of in vitro-induced embryonic stem cells can reconstitute the lymphoid compartments of T- and B-lymphocyte-deficient scid mice and generate mature T and B lymphocytes in sublethally irradiated normal mice. Exogenous cytokines can dramatically alter the developmental fate of embryonic stem cells in culture. The in vitro system described here should facilitate the study of molecular events leading to cell-lineage commitment and to the formation of hemopoietic stem cells and their immediate lymphoid progeny VSports手机版. .

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