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. 2003 Sep 18;425(6955):311-6.
doi: 10.1038/nature01959. Epub 2003 Sep 3.

"V体育官网入口" The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase

Lionel Pintard  1 , John H Willis, Andrew Willems, Jacque-Lynne F Johnson, Martin Srayko, Thimo Kurz, Sarah Glaser, Paul E Mains, Mike Tyers, Bruce Bowerman, Matthias Peter
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The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase

Lionel Pintard et al. Nature. .

Abstract

Many biological processes, such as development and cell cycle progression are tightly controlled by selective ubiquitin-dependent degradation of key substrates. In this pathway, the E3-ligase recognizes the substrate and targets it for degradation by the 26S proteasome VSports手机版. The SCF (Skp1-Cul1-F-box) and ECS (Elongin C-Cul2-SOCS box) complexes are two well-defined cullin-based E3-ligases. The cullin subunits serve a scaffolding function and interact through their C terminus with the RING-finger-containing protein Hrt1/Roc1/Rbx1, and through their N terminus with Skp1 or Elongin C, respectively. In Caenorhabditis elegans, the ubiquitin-ligase activity of the CUL-3 complex is required for degradation of the microtubule-severing protein MEI-1/katanin at the meiosis-to-mitosis transition. However, the molecular composition of this cullin-based E3-ligase is not known. Here we identified the BTB-containing protein MEL-26 as a component required for degradation of MEI-1 in vivo. Importantly, MEL-26 specifically interacts with CUL-3 and MEI-1 in vivo and in vitro, and displays properties of a substrate-specific adaptor. Our results suggest that BTB-containing proteins may generally function as substrate-specific adaptors in Cul3-based E3-ubiquitin ligases. .

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