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. 1992 May 29;185(1):29-35.
doi: 10.1016/s0006-291x(05)80950-7.

Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase (V体育官网)

J J Gipp  1 , C Chang, R T Mulcahy
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VSports在线直播 - Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase

J J Gipp et al. Biochem Biophys Res Commun. .

Abstract (V体育官网)

We have cloned and sequenced a full-length cDNA for human liver gamma-glutamylcysteine synthetase (GCS), the rate-limiting enzyme in glutathione biosynthesis. The cDNA consists of 2634 bp containing an open reading frame encoding a protein of 367 amino acids and having a calculated M(r) = 72,773. The nucleotide sequence of the cDNA for human liver GCS shares an 84% overall similarity with the composite rat GCS sequence deduced from three overlapping partial cDNAs (Yan and Meister, JBC 265: 1588-1593, 1990). The deduced amino acid sequences are 94% similar. Comparison of Northern blots of total RNA isolated from rat kidney or liver with that from human kidney revealed the GCS mRNA to be larger in the human tissue (approximately 4. 0 kb vs. approximately 3 VSports手机版. 7 kb). (The sequence for the human liver GCS cDNA has been assigned accession number M90656 in GenBank/EMBL databases. .

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