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. 2003 Mar;9(3):446-8.
doi: 10.3748/wjg.v9.i3.446.

Protective effect of ascorbic acid in experimental gastric cancer: reduction of oxidative stress

Claudia P M S Oliveira  1 , Paulo Kassab, Fabio P Lopasso, Heraldo P Souza, Mariano Janiszewski, Francisco R M Laurindo, Kioshi Iriya, Antonio A Laudanna
Affiliations

Protective effect of ascorbic acid in experimental gastric cancer: reduction of oxidative stress

Claudia P M S Oliveira et al. World J Gastroenterol. 2003 Mar.

Abstract

Aim: Oxidative stress participates in the cell carcinogenesis by inducing DNA mutations. Our aim was to assess whether ascorbic acid, an antioxidant, could have a role in preventing ROS (Reactive Oxygen Species) generation in experimental gastric carcinoma in a rat model VSports手机版. .

Methods: Experimental gastric cancer was induced in twelve Wistar male rats (weighting 250-350 g) by profound duodeno-gastric reflux throught split gastrojenunostomy. The rats were allocated to the following groups: Group I (n=6) was the control; Group II (n=6) which was mantained with daily intake of tape water with Vitamin C (30 mg/Kg). After 6 or 12 months, samples of gastric tumor or non tumor mucosa were taken from the anastomosis of both groups. Oxidative stress was measured by superoxide quantification through lucigenin-amplified chemiluminescence base and by staining with Nitrobluetetrazolium. The histopathologic confirmation of adenocarcinoma was made by eosin-hemathoxilin method. V体育安卓版.

Results: The intestinal type of gastric adenocarcinoma was microscopically identified in all animals of group I whereas only 3 rats of group II showed an adenocarcinoma without macroscopic evidence of them. The cancers were located in the anastomosis in all cases. Basal luminescence from tumor gastric tissue generated 38. 4+/-6. 8 count per minute/mg/X10(6) (mean+/-SD) and 14. 9+/-4. 0 count per minute/mg/X10(6), respectively, in group I and II animals (P<0. 05). The Nitrobluetetrazolium method showed intense staining in tumor tissues but not in non neoplasic mucosa. V体育ios版.

Conclusion: Experimental gastric tumors seem to produce more reactive oxygen species than non neoplasic gastric tissue. The reduction of oxidative stress and gastric tumor incidence in rats were induced by the intake of ascorbic acid VSports最新版本. Therefore, it may have a role in the prevention of gastric carcinoma. .

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V体育官网入口 - Figures

Figure 1
Figure 1
A. Microscopic appearance of the gastric tumor (Note the tubular pattern with variability of glomerular size, rudimentary papillary and nuclear atipia); B. Macroscopic appearance.
Figure 2
Figure 2
Lucigenin amplified-luminescence (count per minute × 106). Basal Luminescence in the tumor and non tumoral mucosa. The addition of SOD (100 kU/L) to the substrate de-creases the amplified-luminescence in visible tumoral mucosa and non tumoral gastric mucosa of rats.
See this image and copyright information in PMC

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