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. 2002 Oct;46(10):3308-10.
doi: 10.1128/AAC.46.10.3308-3310.2002.

Antimicrobial properties of milk: dependence on presence of xanthine oxidase and nitrite (VSports在线直播)

John T Hancock  1 , Vyv Salisbury, Maria Cristina Ovejero-Boglione, Robert Cherry, Catherine Hoare, Robert Eisenthal, Roger Harrison
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Antimicrobial properties of milk: dependence on presence of xanthine oxidase and nitrite

John T Hancock et al. Antimicrob Agents Chemother. 2002 Oct.

Abstract (V体育官网入口)

Human and bovine milk inhibited the metabolic activity of Escherichia coli, as shown by luminescence monitoring of constructs expressing the luxCDABE genes VSports手机版. Inhibition was dependent on both xanthine oxidase (XO) activity and on the presence of nitrite, implying that XO-generated nitric oxide functions as an antibacterial agent. .

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Figures

FIG. 1.
FIG. 1.
The effects of bovine milk and human milk on the metabolic rates of E. coli are mediated by XO. E. coli isolate 16906, transformed with the luxCDABE operon, was resuspended in a luminometer cuvette under conditions of 0.63% oxygen as described in the text. Pterin (10 μM) and sodium nitrite (20 mM) were added, and subsequent light emissions were recorded before and after the addition of 1 ml of bovine (A) or human (B) milk. Measurements were repeated after pretreatment of milk with 25 μM oxypurinol. The traces shown are representative of at least six experiments.
FIG. 2.
FIG. 2.
The antibacterial activity of human milk requires nitrite. E. coli isolate 16906, transformed with the luxCDABE operon, was resuspended in a luminometer cuvette under conditions of 0.63% oxygen as described in the text. Pterin (10 μM) and sodium nitrite (20 mM) were added, and subsequent light emissions were recorded before and after the addition of 1 ml of human milk. Measurements were repeated in the absence of nitrite.
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"V体育平台登录" References

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