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. 2002 Feb;70(2):762-70.
doi: 10.1128/IAI.70.2.762-770.2002.

Rgg influences the expression of multiple regulatory loci to coregulate virulence factor expression in Streptococcus pyogenes

Michael S Chaussee  1 , Gail L Sylva, Daniel E Sturdevant, Laura M Smoot, Morag R Graham, Robert O Watson, James M Musser
Affiliations

VSports最新版本 - Rgg influences the expression of multiple regulatory loci to coregulate virulence factor expression in Streptococcus pyogenes

Michael S Chaussee (VSports注册入口) et al. Infect Immun. 2002 Feb.

Abstract (V体育平台登录)

The human pathogen Streptococcus pyogenes secretes many proteins to the cell wall and extracellular environment that contribute to virulence. Rgg regulates the expression of several exoproteins including a cysteine protease (SPE B), a nuclease (MF-1), a putative nuclease (MF-3), and autolysin. The functional heterogeneity of Rgg-regulated exoproteins and the lack of a conserved regulatory motif in the promoter regions of the genes suggested that Rgg interacts with additional regulatory networks to influence gene expression. DNA microarrays were used to test this hypothesis by comparing genomewide transcript profiles of S. pyogenes NZ131 and isogenic derivative NZ131 rgg during the exponential phase of growth VSports手机版. Transcripts of known and putative virulence-associated genes were more abundant in the rgg mutant, including emm, scpA, orfX, scl1, hasAB, slo, sagA, ska, speH, grab, mac, mf-1, and mf-3. Increased transcription of emm, scpA, and orfX in the rgg mutant was associated with increased production of the corresponding proteins. Differences in the expression of virulence-associated genes were associated with changes in the expression of several regulatory genes, including mga, sagA, csrRS, and fasBCA. The results show that Rgg influences the expression of multiple regulatory networks to coregulate virulence factor expression in S. pyogenes. .

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Figures

FIG. 1.
FIG. 1.
Increased transcription of the Mga regulon in NZ131 rgg. Total RNA was isolated from exponential-phase cultures of wild-type (wt) NZ131, speB mutant strain NZ131, and rgg mutant strain NZ131, and the quantities of gene-specific transcripts were determined by quantitative RT-PCR. The quantity of cDNA for each gene was normalized to the quantity of gyrA cDNA in each RNA sample. The values shown are the mean ± the standard error of the mean of at least two independently isolated RNA preparations analyzed in triplicate.
FIG. 2.
FIG. 2.
Increased production of M protein, C5a peptidase (SCP), and OrfX in NZ131 rgg. Production of selected Mga-regulated proteins was assessed by immunoblotting following whole-cell electrophoresis of wild-type (wt) strain NZ131 (lane 1), speB mutant strain NZ131 (lane 2), and rgg mutant strain NZ131 (lane 3). M protein (αM49), C5a peptidase (αSCP), and OrfX (αOrfX) were detected with antisera specific to the polypeptides, as indicated to the left of each panel. The migration and size of the molecular mass standards are shown to the right of each panel. Antisera to the group A streptococcus carbohydrate (αGAS) was used to confirm that similar amounts of cell wall antigens were analyzed. The experiment was repeated twice, and a representative result is shown.
FIG. 3.
FIG. 3.
Influence of Rgg with other regulatory networks. Positive regulation and negative regulation of gene expression are designated by plus and minus signs, respectively. Dashed arrows indicate possible indirect influences on gene expression. Dashed ovals represent putative regulatory intermediates.
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References (V体育安卓版)

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