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. 2001 Oct;69(10):6179-85.
doi: 10.1128/IAI.69.10.6179-6185.2001.

"V体育2025版" TonB-dependent systems of uropathogenic Escherichia coli: aerobactin and heme transport and TonB are required for virulence in the mouse

A G Torres  1 , P Redford, R A Welch, S M Payne
Affiliations

TonB-dependent systems of uropathogenic Escherichia coli: aerobactin and heme transport and TonB are required for virulence in the mouse

A G Torres et al. Infect Immun. 2001 Oct.

Abstract

The uropathogenic Escherichia coli strain CFT073 has multiple iron acquisition systems, including heme and siderophore transporters. A tonB mutant derivative of CFT073 failed to use heme as an iron source or to utilize the siderophores enterobactin and aerobactin, indicating that transport of these compounds in CFT073 is TonB dependent. The TonB(-) derivative showed reduced virulence in a mouse model of urinary tract infection. Virulence was restored when the tonB gene was introduced on a plasmid. To determine the importance of the individual TonB-dependent iron transport systems during urinary tract infections, mutants defective in each of the CFT073 high-affinity iron transport systems were constructed and tested in the mouse model. Mouse virulence assays indicated that mutants defective in a single iron transport system were able to infect the kidney when inoculated as a pure culture but were unable to efficiently compete with the wild-type strain in mixed infections VSports手机版. These results indicate a role for TonB-dependent systems in the virulence of uropathogenic E. coli strains. .

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"V体育2025版" Figures

FIG. 1
FIG. 1
Recovery of bacteria from the kidneys of mice infected with TonB+ and TonB− strains. Quantitative bacterial counts are shown. Each X symbol represents CFU per gram of tissue from an individual mouse. Values near the x axes indicate no detectable bacteria in the kidney.
See this image and copyright information in PMC

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