Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official VSports app下载. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely V体育官网. .

. 2000 Jul;68(7):4145-54.
doi: 10.1128/IAI.68.7.4145-4154.2000.

Relationship between the Tsh autotransporter and pathogenicity of avian Escherichia coli and localization and analysis of the Tsh genetic region

C M Dozois  1 , M Dho-Moulin, A Brée, J M Fairbrother, C Desautels, R Curtiss 3rd
Affiliations

Relationship between the Tsh autotransporter and pathogenicity of avian Escherichia coli and localization and analysis of the Tsh genetic region

C M Dozois et al. Infect Immun. 2000 Jul.

Abstract (V体育ios版)

The temperature-sensitive hemagglutinin Tsh is a member of the autotransporter group of proteins and was first identified in avian-pathogenic Escherichia coli (APEC) strain chi7122. The prevalence of tsh was investigated in 300 E. coli isolates of avian origin and characterized for virulence in a 1-day-old chick lethality test. Results indicate that among the tsh-positive APEC isolates, 90. 6% belonged to the highest virulence class. Experimental inoculation of chickens with chi7122 and an isogenic tsh mutant demonstrated that Tsh may contribute to the development of lesions within the air sacs of birds but is not required for subsequent generalized infection manifesting as perihepatitis, pericarditis, and septicemia. Conjugation and hybridization experiments revealed that the tsh gene is located on a ColV-type plasmid in many of the APEC strains studied, including strain chi7122, near the colicin V genes in most of these strains. DNA sequences flanking the tsh gene of strain chi7122 include complete and partial insertion sequences and phage-related DNA sequences, some of which were also found on virulence plasmids and pathogenicity islands present in various E. coli pathotypes and other pathogenic members of the Enterobacteriaceae VSports手机版. These results demonstrate that the tsh gene is frequently located on the ColV virulence plasmid in APEC and suggest a possible role of Tsh in the pathogenicity of E. coli for chickens in the early stages of infection. .

PubMed Disclaimer

"VSports在线直播" Figures

FIG. 1
FIG. 1
Restriction maps and genetic organization of (a) the tsh gene region and (b) the colicin V (ColV) gene cluster. Organization of the ColV gene cluster is derived from Gilson et al. (27). Genes are indicated below the restriction maps with thick black arrows that point in the direction of transcription. Forward (>) and reverse (<) primers for PCR amplifications of DNA from avian E. coli isolates and synthesis of DNA probes are indicated with numbers. Oligonucleotide primers are described in Table 2. DNA probes Tsh1 (620 bp), Tsh2 (616 bp), and ColV1 (1,203 bp) are indicated with solid lines above the restriction maps. The Tsh1 and Tsh2 probes were generated from plasmid pYA3108 (54), and the ColV1 probe was generated from pHK11 (27). Experimental procedures are detailed in the text.
FIG. 2
FIG. 2
(A) Localization of tsh to ColV-type plasmids in APEC isolates and pColV-K30. Analysis of plasmids of tsh-positive APEC isolates, χ7122, and derivatives by ethidium bromide staining (upper panels) and Southern hybridization of plasmid extracts (lower panels). Asterisks indicate samples from ColV-negative isolates obtained by PCR. Hybridizations depicted are with the Tsh1 probe. Hybridization with the ColV1 probe is marked below the lower panels as positive (+) or negative (−). In positive samples, the ColV1 probe hybridized with the same plasmid as the Tsh1 probe. Arrows indicate the pAPEC-1 plasmid containing tsh in APEC strain χ7122. Lane 1, χ7273; lane 2, χ7274; lanes 3 and 11, pColV-K30; lanes 4 and 12, χ7122; lane 5, TK27; lane 6, CN30; lane 7, TK40; lane 8, TK60; lane 9, CN137; lane 10, CN139; lane 13, CN144; lane 14, CN151; lane 15, CN163; lane 16, CN165; lane 17, CN69; lane 18, TK49; lane 19, CN14; lane 20, CN71. CN (isolated from chicken) and TK (isolated from turkey) samples are clinical isolates from Québec, Canada. (B) Replacement of plasmid pAPEC-1 from strain χ7122 with a Tn10-tagged pColV-K30 plasmid; plasmid profiles of donor, recipient, and transconjugant strains. Lane 1, donor strain MEG-617(pColV-K30::lacZ); lane 2, recipient χ7122 native plasmid profile before conjugation; lane 3, strain χ7275, a transconjugant of χ7122 mated with MEG-617(pColV-K30::lacZ) following selection on medium containing tetracycline and nalidixic acid.
FIG. 3
FIG. 3
Effect of 90% normal chicken serum on survival of APEC strain χ7122 and derivatives and E. coli K-12 with and without the pAPEC-1 plasmid. Strains: χ7122, APEC wild-type strain; χ7273, χ7122 tsh::tetAR; χ7274, χ7273 ΔpAPEC-1; χ7276, E. coli K-12; and χ7277, χ7276(pAPEC-1). Results are from a representative experiment from three independent assays.
FIG. 4
FIG. 4
Organization and analysis of the tsh region of pAPEC-1 from strain χ7122. The scale is given in kilobase pairs. ORFs are represented by arrows above the scale. Solid arrows represent complete coding (black arrows) or interrupted (grey arrow) sequences showing high identity to known genes or IS elements. Putative ORFs are represented by hatched arrows. Positions and predicted lengths of the ORF products are presented in Table 5. Horizontal lines below the scale represent sequences exhibiting nucleotide identity to the specified region. The percent nucleotide identity and accession numbers of sequences exhibiting nucleotide identity to the represented regions are as follows: IS91, 100% (K04543); IS100, 97% (Z32853); IS30, 100% (X00792); “pColV-K30” of E. coli EB-1, 99.8% (AJ223631); IS911, 100% (X17613); bacteriophage N15, 79% (AF064539); IS1294, 92% (X82430); def (fms), 97% (X63666); prophage 933L, 99% (AF097644); she PAI, 97% (U97493); pic, 100% (AF097644); and pssA, 98% (Y13614).
See this image and copyright information in PMC

References

    1. Alexeyev M F. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques. 1995;18:52–56. - PubMed
    1. Altschul S F, Madden T L, Schaffer A A, Zhang J, Zhang Z, Miller W, Lipman D J. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997;25:3389–3402. - PMC (VSports在线直播) - PubMed
    1. Ausubel F M, Brent R, Kingston R E, Moore D M, Seidman J G, Smith J A, Struhl K, editors. Current protocols in molecular biology. New York, N.Y: Greene Publishing Associates and Wiley-Interscience; 1991.
    1. Bagg A, Neilands J B. Mapping of a mutation affecting regulation of iron uptake systems in Escherichia coli K-12. J Bacteriol. 1985;161:450–453. - PMC - PubMed
    1. Benjelloun-Touimi Z, Sansonetti P J, Parsot C. SepA, the major extracellular protein of Shigella flexneri: autonomous secretion and involvement in tissue invasion. Mol Microbiol. 1995;17:123–135. - PubMed

"VSports" Publication types

MeSH terms

Associated data

"VSports手机版" LinkOut - more resources