<font draggable="Bd5p4Yk"></font><var lang="GQtoR8"><style lang="xALgnCzH"></style></var> Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 1999 Jun 7;189(11):1777-82.
doi: 10.1084/jem.189.11.1777.

MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4

R Shimazu  1 , S Akashi, H Ogata, Y Nagai, K Fukudome, K Miyake, M Kimoto
Affiliations

MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4

R Shimazu et al. J Exp Med. .

Abstract

Toll-like receptor 4 (TLR4) is a mammalian homologue of Drosophila Toll, a leucine-rich repeat molecule that can trigger innate responses against pathogens. The TLR4 gene has recently been shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to lipopolysaccharide (LPS). TLR4 may be a long-sought receptor for LPS. However, transfection of TLR4 does not confer LPS responsiveness on a recipient cell line, suggesting a requirement for an additional molecule. Here, we report that a novel molecule, MD-2, is requisite for LPS signaling of TLR4. MD-2 is physically associated with TLR4 on the cell surface and confers responsiveness to LPS. MD-2 is thus a link between TLR4 and LPS signaling. Identification of this new receptor complex has potential implications for understanding host defense, as well as pathophysiologic, mechanisms VSports手机版. .

PubMed Disclaimer

Figures

Figure 1
Figure 1
The nucleotide sequence of human MD-2 and its similarity to human MD-1. (A) The nucleotide sequence is shown with a deduced amino acid sequence. The signal peptide and canonical N-glycosylation sites are underlined. The stop codon is denoted by an asterisk. The nucleotide sequence of MD-2 will appear in the EMBL/GenBank/DDBJ nucleotide sequence databases under accession no. AB018549. (B) Amino acid sequences of human MD-1 and MD-2 were aligned using the BLAST2 program at the National Center for Biological Information (Bethesda, MD).
Figure 2
Figure 2
Expression of the MD-2 transcript. (A) Total RNA (20 μg/lane) from the indicated human cell lines was electrophoresed and hybridized with probes for human MD-2, TLR4, and GAPDH as indicated. Nalm-6, Ramos, Daudi, and RPMI8866 are of B lymphocyte lineage. CEM and Molt4 are of T cell origin. U937 and THP-1 are of monocytic origin. K562 is the erythroleukemia line. (B) Total RNA (20 μg/lane) from the indicated mouse tissues was used for Northern hybridization. The cDNA clone encoding mouse MD-2 was used as a probe. For GAPDH, the rat cDNA clone was used.
Figure 2
Figure 2
Expression of the MD-2 transcript. (A) Total RNA (20 μg/lane) from the indicated human cell lines was electrophoresed and hybridized with probes for human MD-2, TLR4, and GAPDH as indicated. Nalm-6, Ramos, Daudi, and RPMI8866 are of B lymphocyte lineage. CEM and Molt4 are of T cell origin. U937 and THP-1 are of monocytic origin. K562 is the erythroleukemia line. (B) Total RNA (20 μg/lane) from the indicated mouse tissues was used for Northern hybridization. The cDNA clone encoding mouse MD-2 was used as a probe. For GAPDH, the rat cDNA clone was used.
Figure 3
Figure 3
Membrane anchoring of MD-2 via TLR4. A stable line expressing MD-2 with the flag epitope was stained with the anti-flag mAb, followed by goat anti–mouse IgG–FITC (a and d). Staining with cell permeabilization is shown in d. Another stable transfectant was used that expressed MD-2 with the protein C epitope and TLR4 in b, c, and e. Cell surface MD-2 and TLR4 are shown with the rat anti–protein C mAb or the mouse anti–human TLR4 mAb HTA125 (b and c, respectively). MD-2 expression in permeabilized cells is shown in e. The second reagents used were goat anti–rat IgG–PE or goat anti–mouse IgG–FITC, respectively. Control histograms stained with isotype-matched mouse or rat mAb are shown (dotted lines).
Figure 4
Figure 4
MD-2 is coprecipitated with TLR4. (A) Stable transfectants expressing MD-2 alone or with TLR4 were stained with or without cell permeabilization. The intracellular MD-2 precursor was stained with the anti-protein C mAb. The specificity of the anti-TLR4 mAb was shown by cell surface staining of stable transfectants. Goat anti– mouse IgG–FITC was used as the second reagent. Dotted lines correspond to histograms stained with the second reagent alone. Panels correspond to the original Ba/F3 line, a transfectant expressing TLR4 alone, and a transfectant expressing TLR4 + MD-2, respectively. (B) Stable transfectants expressing TLR4 and MD-2 were subjected to immunoprecipitation with control mouse IgG (lane 1) or the anti-TLR4 mAb, HTA125 (lanes 2 and 3). After blotting, precipitated molecules were detected with an anti-flag mAb, M2. The precipitates shown in lanes 1 and 2 are from the transfectant expressing TLR4 and MD-2, both of which were tagged with the flag epitope. In lane 3, we used the control line in which the flag epitope was on TLR4 but not on MD-2.
Figure 4
Figure 4
MD-2 is coprecipitated with TLR4. (A) Stable transfectants expressing MD-2 alone or with TLR4 were stained with or without cell permeabilization. The intracellular MD-2 precursor was stained with the anti-protein C mAb. The specificity of the anti-TLR4 mAb was shown by cell surface staining of stable transfectants. Goat anti– mouse IgG–FITC was used as the second reagent. Dotted lines correspond to histograms stained with the second reagent alone. Panels correspond to the original Ba/F3 line, a transfectant expressing TLR4 alone, and a transfectant expressing TLR4 + MD-2, respectively. (B) Stable transfectants expressing TLR4 and MD-2 were subjected to immunoprecipitation with control mouse IgG (lane 1) or the anti-TLR4 mAb, HTA125 (lanes 2 and 3). After blotting, precipitated molecules were detected with an anti-flag mAb, M2. The precipitates shown in lanes 1 and 2 are from the transfectant expressing TLR4 and MD-2, both of which were tagged with the flag epitope. In lane 3, we used the control line in which the flag epitope was on TLR4 but not on MD-2.
Figure 5
Figure 5
MD-2 enhances ligand-independent signaling via TLR4. A human kidney cell line, 293T, was transfected with expression vectors encoding the molecules indicated. A reporter plasmid for NF-κB activity and an expression vector encoding β-galactosidase was also transfected (see Materials and Methods). Relative NF-κB activity was calculated by normalizing luciferase activity with β-galactosidase activity. Data are shown as mean values from triplicate wells. Error bars, SD.
Figure 6
Figure 6
MD-2 confers LPS signaling on TLR4. (A) Stable transfectants (see Materials and Methods for details) expressing TLR4 alone (▪) or TLR4 and MD-2 (•) were stimulated with LPS from E. coli 055:B5, S. minnesota Re595, or lipid A at the concentrations indicated. After a 4-h culture, cells were harvested, and luciferase activity was determined and expressed as relative light units. (B) The transfectant expressing TLR4 and MD-2 was stimulated with LPS from E. coli 055: B5, LPS from S. minnesota Re595, or lipid A at 100 ng/ml. The mAb HTA125 (black bars) or a control mAb (gray bars) were included in the indicated groups (10 μg/ml). After 4 h, NF-κB activity was determined. Data represent mean values from triplicate wells.
Figure 6
Figure 6
MD-2 confers LPS signaling on TLR4. (A) Stable transfectants (see Materials and Methods for details) expressing TLR4 alone (▪) or TLR4 and MD-2 (•) were stimulated with LPS from E. coli 055:B5, S. minnesota Re595, or lipid A at the concentrations indicated. After a 4-h culture, cells were harvested, and luciferase activity was determined and expressed as relative light units. (B) The transfectant expressing TLR4 and MD-2 was stimulated with LPS from E. coli 055: B5, LPS from S. minnesota Re595, or lipid A at 100 ng/ml. The mAb HTA125 (black bars) or a control mAb (gray bars) were included in the indicated groups (10 μg/ml). After 4 h, NF-κB activity was determined. Data represent mean values from triplicate wells.
See this image and copyright information in PMC

References (V体育ios版)

    1. Hammond-Kosak KE, Jones JDG. Plant disease resistance genes. Annu Rev Plant Physiol Plant Mol Biol. 1997;48:575–607. - PubMed
    1. Lemaitre B, Nicolas E, Michaut L, Reichhart J-M, Hoffmann JA. The dorsoventral regulatory gene cassette spaetzle/Toll/cactus controls the potent antifungal response in Drosophilaadults. Cell. 1996;86:973–983. - PubMed (VSports app下载)
    1. Williams MJ, Rodliguez A, Kimbrell DA, Eldon ED. The 18-wheeler mutation reveals complex antibacterial gene regulation in Drosophilahost defense. EMBO (Eur Mol Biol Organ) J. 1997;20:6120–6130. - VSports手机版 - PMC - PubMed
    1. Medzhitov R, Janeway CA., Jr Innate immunity: impact on the adaptive immune response. Curr Opin Immunol. 1997;9:4–9. - PubMed
    1. Vogel G. Fly development genes lead to immune find. Science. 1998;281:1942–1944. - V体育平台登录 - PubMed

Publication types

MeSH terms

Associated data