"VSports最新版本" PRINT

BCL2-like 2

Antibody: CPTC-BCL2L2-1
Background

Catalog Number:

CPTC-BCL2L2-1

RRID:

AB_1553418

Target Antigen:

BCL2-like 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

02/26/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (111.2 KB)

CPTC-BCL2L2-1 IHC evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www V体育官网. proteinatlas. org).


Characterization SOP Files (V体育官网)

  IHC NCI60

CPTC-BCL2L2-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


VSports最新版本 - Characterization SOP Files

  IHC Tissue

V体育官网 - CPTC-BCL2L2-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files (V体育2025版)

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

"V体育安卓版" CPTC-BCL2L2-1 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NAPPA
Download This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique. (1.4 MB)

"VSports app下载" CPTC-BCL2L2-1 Evaluation of antibody by NAPPA

Result: Positive

This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique. VSports在线直播.


Characterization SOP Files

  NCI 60 Protein Array

V体育平台登录 - CPTC-BCL2L2-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


V体育官网入口 - Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-BCL2L2-1 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-BCL2L2-1 Western Blot (Recombinant Protein)

Result: Positive

Western Blot using CPTC-BCL2L2-1 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3) V体育官网.


Characterization SOP Files

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Antibody: CPTC-BCL2L2-2
Background

Catalog Number:

CPTC-BCL2L2-2

RRID:

AB_10570545

Target Antigen:

BCL2-like 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

02/26/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on ovarian cancer tissue core using CPTC-BCL2L2-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (pancreas, lung, testis, endometrium, and kidney) and cancer tissues (colon, ovarian, lung, and prostate). Click image to enlarge

CPTC-BCL2L2-2 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on ovarian cancer tissue core using CPTC-BCL2L2-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0. 5mg/mL stock V体育安卓版. Signal was also obtained in other normal tissues (pancreas, lung, testis, endometrium, and kidney) and cancer tissues (colon, ovarian, lung, and prostate).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (127.3 KB)

CPTC-BCL2L2-1 IHC evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www. proteinatlas. org) VSports最新版本.


Characterization SOP Files

  IHC NCI60

CPTC-BCL2L2-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

V体育官网 - CPTC-BCL2L2-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

V体育平台登录 - CPTC-BCL2L2-2 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files (V体育官网入口)

  NCI 60 Protein Array

CPTC-BCL2L2-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


V体育官网入口 - Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-BCL2L2-2 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-BCL2L2-2 Western Blot (Recombinant Protein)

Result: Positive

Western Blot using CPTC-BCL2L2-2 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3) V体育安卓版.


Characterization SOP Files

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Antibody: CPTC-BCL2L2-3
Background

Catalog Number:

CPTC-BCL2L2-3

RRID:

AB_2228095

Target Antigen:

BCL2-like 2

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

02/26/2025

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (105.4 KB)

CPTC-BCL2L2-3 IHC evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www. proteinatlas. org) VSports最新版本.


Characterization SOP Files (V体育ios版)

  IHC NCI60

CPTC-BCL2L2-3 IHC NCI60 (VSports最新版本)

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-BCL2L2-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-BCL2L2-3 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NAPPA
Download This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique. (689.6 KB)

CPTC-BCL2L2-3 Evaluation of antibody by NAPPA

Result: Positive

This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique V体育官网. .


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BCL2L2-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-BCL2L2-3 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-BCL2L2-3 Western Blot (Recombinant Protein) (V体育ios版)

Result: Positive

Western Blot using CPTC-BCL2L2-3 as primary Ab against Ag 10179 (lane 2). Also included are molecular wt VSports最新版本. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: BCL2-like 2
Background

NCI Identification Number:

10179

Antigen Name:

BCL2-like 2

CPTC Name:

CPTC-BCL2L2

Aliases:

BCL2L2; BCL-W; BCLW; KIAA0271

Function:

This gene encodes a pro-survival (anti-apoptotic) member of the bcl-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis. Relative to its Bcl-2 counterparts there is considerably less data on this particular protein however much effort is being made to further understand its cellular roles. Located on chromosome 14q11 it appears to be redundant in most tissues apart from specific examples. It was originally discovered by Gibson et al. Gibson L, Holmgreen SP, Huang DC, et al VSports注册入口. (1996). "bcl-w, a novel member of the bcl-2 family, promotes cell survival. ". Oncogene 13 (4): 665 .

Chromosomal Localization:

14q11.2 - q12

Expression System:

E. Coli

Accession Number:

NM_004050

UniProt Accession Number:

Q92843

DNA Source:

HIP:HsCD00000436

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

33523

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMR
AAGDEFETRFRRTFSDLAAQLHVTPGSAQQRFTQVSDELFQGGPNWGRLV
AFFVFGAALCAESVNKEMEPLVGQVQEWMVAYLETRLADWIHSSGGWAEF
TALYGDGALEEARRLREGNWA

Native Sequence:

MATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMRAAG
DEFETRFRRTFSDLAAQLHVTPGSAQQRFTQVSDELFQGGPNWGRLVAFF
VFGAALCAESVNKEMEPLVGQVQEWMVAYLETRLADWIHSSGGWAEFTAL
YGDGALEEARRLREGNWASVRTVLTGAVALGALVTVGAFFASK

Calculated Isoelectric Point:

5.06

Molecular Weight:

18629

Last Updated:

09/01/2008

"VSports注册入口" Links

Characterization Data

Gel

Click to enlarge image PAGE of Ag 10179 (with molecular weight standards in lane 1)
Click image to enlarge

Ag 10179

PAGE of Ag 10179 (with molecular weight standards in lane 1)

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SOPs (V体育安卓版)

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